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Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cells for FeFAB assay were kind gifts from Dr Martin Löchelt, German Cancer Research Center, Germany.

The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

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